Opsonization and Phagocytosis of Plasmodium falciparum Merozoites Measured by Flow Cytometry
نویسندگان
چکیده
منابع مشابه
Opsonization and phagocytosis of Plasmodium falciparum merozoites measured by flow cytometry.
A flow cytometric phagocytosis assay was established to investigate the role of anti-merozoite antibody, complement, and cytokines on the phagocytosis of Plasmodium falciparum merozoites by human neutrophils. This assay involved allowing fluorescein isothiocyanate-labeled merozoites to interact with phagocytes and analysis of the cells on a FACScan with Lysis II software. To differentiate the p...
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Two Nigerian siblings, ages 10 and 4 years, respectively, were infected with Plasmodium falciparum and were admitted to the hospital on the same day. The younger child died on the day of admission, but the older child survived. The peripheral blood smears of the younger patient showed the ring forms, schizonts, free merozoites, and phagocytosis of malarial parasites by both monocytes and polymo...
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There is intense interest in induction and characterization of strain-transcending neutralizing Ab against antigenically variable human pathogens. We have recently identified the human malaria parasite Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) as a target of broadly neutralizing Abs, but there is little information regarding the functional mechanism(s) of Ab-mediated ...
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BACKGROUND An understanding of the mechanisms mediating protective immunity against malaria in humans is currently lacking, but critically important to advance the development of highly efficacious vaccines. Antibodies play a key role in acquired immunity, but the functional basis for their protective effect remains unclear. Furthermore, there is a strong need for immune correlates of protectio...
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ژورنال
عنوان ژورنال: Clinical Diagnostic Laboratory Immunology
سال: 2000
ISSN: 1071-412X,1098-6588
DOI: 10.1128/cdli.7.1.9-13.2000